Coding

Part:BBa_K2092000:Design

Designed by: Guadalupe Alvarez Gonzalez   Group: iGEM16_Manchester   (2016-10-11)


AOx


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 363
    Illegal BglII site found at 540
    Illegal BamHI site found at 1305
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 595
    Illegal AgeI site found at 1045
    Illegal AgeI site found at 1173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is designed with an NdeI restriction site between the prefix and the start of the AOx gene. This is because we wanted to assemble the gene in protein expression pET vectors, which normally contain an NdeI restriction site after the Ribosome Binding Site.


Source

GenBank

References